Peel Road, between Quarterbridge and Braddan Bridge will shut overnight tonight and tomorow.
The emergency road closure will see the route closed from 6.30pm until 6am over the next couple of days.
The DOI says it's while the stretch is resurfaced ahead of this year's TT.
The department also says the surface of the road has 'recently deteriorated significantly' because of icy conditions earlier this year.
It says resurfacing is "necessary for both everyday traffic on one of the Island's busiest roads but also to allow TT racing to go ahead."
DIVERSIONS:
The road between Braddan Bridge and Quarterbridge will be closed to through traffic by motor vehicles.
Access to Douglas Rugby Club will be available from the Braddan Bridge end of the closure
Spring Valley Road between Saddle Road and Quarterbridge will be closed to motor vehicles (pedestrians will be unaffected)
TRAFFIC TO/FROM THE WEST
• Traffic will be diverted at Saddle Road; with alternative routes being Strang Road or Braddan Road
TRAFFIC TO/FROM SOUTH AND WEST
• Will be diverted between Spring Valley Road and Peel Road, Braddan via Saddle Road
TRAFFIC BETWEEN DOUGLAS & THE NORTH WITH THE SOUTH
• Will be routed via Peel Road/Pulrose Bridge/Groves Road/Spring Valley Road